1i3f

X-ray diffraction
2.35Å resolution

Ribonuclease T1 V89S mutant

Released:
Source organism: Aspergillus oryzae
Primary publication:
Hydrophobic core manipulations in ribonuclease T1.
Biochemistry 40 10140-9 (2001)
PMID: 11513591

Function and Biology Details

Reaction catalysed:
(1a) (RNA) containing guanosine = (RNA fragment)-3'-guanosine-2',3'-cyclophosphate + a 5'-hydroxy-ribonucleotide-3'-(RNA fragment)
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Guanyl-specific ribonuclease T1 Chain: A
Molecule details ›
Chain: A
Length: 104 amino acids
Theoretical weight: 11.08 KDa
Source organism: Aspergillus oryzae
Expression system: Escherichia coli
UniProt:
  • Canonical: P00651 (Residues: 27-130; Coverage: 100%)
Gene names: AO090011000118, rntA
Structure domains: Microbial ribonucleases

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X11
Spacegroup: P212121
Unit cell:
a: 40.308Å b: 46.886Å c: 49.901Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.19 0.173 0.283
Expression system: Escherichia coli