1i3a

X-ray diffraction
2.15Å resolution

RNASE HII FROM ARCHAEOGLOBUS FULGIDUS WITH COBALT HEXAMMINE CHLORIDE

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribonuclease HII Chain: A
Molecule details ›
Chain: A
Length: 225 amino acids
Theoretical weight: 25.39 KDa
Source organism: Archaeoglobus fulgidus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O29634 (Residues: 1-205; Coverage: 100%)
Gene names: AF_0621, rnhB
Sequence domains: Ribonuclease HII
Structure domains:

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: P6522
Unit cell:
a: 74.555Å b: 74.555Å c: 139.684Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.223 0.223 0.272
Expression system: Escherichia coli BL21(DE3)