PDBe 1i27

X-ray diffraction
1.02Å resolution

CRYSTAL STRUCTURE OF THE C-TERMINAL DOMAIN OF THE RAP74 SUBUNIT OF HUMAN TRANSCRIPTION FACTOR IIF (TFIIF)

Released:
Source organism: Homo sapiens

Function and Biology Details

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
General transcription factor IIF subunit 1 Chain: A
Molecule details ›
Chain: A
Length: 73 amino acids
Theoretical weight: 8.37 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P35269 (Residues: 449-517; Coverage: 13%)
Gene names: GTF2F1, RAP74
Structure domains: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P212121
Unit cell:
a: 29.052Å b: 43.024Å c: 48.161Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.127 not available 0.146
Expression system: Escherichia coli BL21(DE3)