1hzg

X-ray diffraction
1.86Å resolution

CRYSTAL STRUCTURE OF THE INACTIVE C866S MUTANT OF THE CATALYTIC DOMAIN OF E. COLI CYTOTOXIC NECROTIZING FACTOR 1

Released:
Source organism: Escherichia coli

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cytotoxic necrotizing factor 1 Chain: A
Molecule details ›
Chain: A
Length: 295 amino acids
Theoretical weight: 32.64 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q47106 (Residues: 720-1014; Coverage: 29%)
Gene name: cnf1
Sequence domains: Rho-activating domain of cytotoxic necrotizing factor
Structure domains: Cytotoxic necrotizing factor, Rho-activating domain

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-5
Spacegroup: P65
Unit cell:
a: 54.9Å b: 54.9Å c: 176.8Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.2 0.198 0.22
Expression system: Escherichia coli BL21(DE3)