1hyr

X-ray diffraction
2.7Å resolution

CRYSTAL STRUCTURE OF HUMAN MICA IN COMPLEX WITH NATURAL KILLER CELL RECEPTOR NKG2D

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
NKG2-D type II integral membrane protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 137 amino acids
Theoretical weight: 15.85 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P26718 (Residues: 80-216; Coverage: 63%)
Gene names: D12S2489E, KLRK1, NKG2D
Sequence domains: Lectin C-type domain
Structure domains: Mannose-Binding Protein A, subunit A
MHC class I polypeptide-related sequence A Chain: C
Molecule details ›
Chain: C
Length: 275 amino acids
Theoretical weight: 31.73 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q29983 (Residues: 24-297; Coverage: 76%)
Gene names: MICA, PERB11.1
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P4212
Unit cell:
a: 122.78Å b: 122.78Å c: 101.82Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.227 0.227 0.282
Expression systems:
  • Escherichia coli BL21(DE3)
  • Escherichia coli