1hyh

X-ray diffraction
2.2Å resolution

CRYSTAL STRUCTURE OF L-2-HYDROXYISOCAPROATE DEHYDROGENASE FROM LACTOBACILLUS CONFUSUS AT 2.2 ANGSTROMS RESOLUTION-AN EXAMPLE OF STRONG ASYMMETRY BETWEEN SUBUNITS

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
L-2-hydroxyisocaproate dehydrogenase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 309 amino acids
Theoretical weight: 33.08 KDa
Source organism: Weissella confusa
Expression system: Escherichia coli
UniProt:
  • Canonical: P14295 (Residues: 2-310; Coverage: 100%)
Sequence domains: lactate/malate dehydrogenase, NAD binding domain
Structure domains:

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MPG/DESY, HAMBURG BEAMLINE BW6
Spacegroup: P3221
Unit cell:
a: 135.9Å b: 135.9Å c: 205.9Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.21 not available 0.25
Expression system: Escherichia coli