› 1hyh

X-ray diffraction
2.2Å resolution

CRYSTAL STRUCTURE OF L-2-HYDROXYISOCAPROATE DEHYDROGENASE FROM LACTOBACILLUS CONFUSUS AT 2.2 ANGSTROMS RESOLUTION-AN EXAMPLE OF STRONG ASYMMETRY BETWEEN SUBUNITS

Released:
DOI: 10.2210/pdb1hyh/pdb
PDB 1hyh coloured by chain and viewed from the front.
PDB 1hyh coloured by chain and viewed from the side.
PDB 1hyh coloured by chain and viewed from the top.
Source organism: Weissella confusa
Primary publication:
Crystal structure of L-2-hydroxyisocaproate dehydrogenase from Lactobacillus confusus at 2.2 A resolution. An example of strong asymmetry between subunits.
Niefind K, Hecht HJ, Schomburg D
J. Mol. Biol. 251 256-81 (1995)
PMID: 7643402

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
L-2-hydroxyisocaproate dehydrogenase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 309 amino acids
Theoretical weight: 33.08 KDa
Source organism: Weissella confusa
Expression system: Escherichia coli
UniProt:
  • Canonical: P14295 (Residues: 2-310; Coverage: 100%)
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand SO4 4 x SO4
Ligand NAD 4 x NAD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MPG/DESY, HAMBURG BEAMLINE BW6
Spacegroup: P3221
Unit cell:
a: 135.9Å b: 135.9Å c: 205.9Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.21 not available 0.25
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Comparative genomics of enzymes in flavor-forming pathways from amino acids in lactic acid bacteria.
Liu et al. (2008)
2 more

1 mention without citation

An Ancient Fingerprint Indicates the Common Ancestry of Rossmann-Fold Enzymes Utilizing Different Ribose-Based Cofactors.
Laurino et al. (2016)