› 1hw2

X-ray diffraction
3.25Å resolution

FADR-DNA COMPLEX: TRANSCRIPTIONAL CONTROL OF FATTY ACID METABOLISM IN ECHERICHIA COLI

Released:
DOI: 10.2210/pdb1hw2/pdb
PDB entry 1hw2 coloured by chain, front view.
PDB entry 1hw2 coloured by chain, side view.
PDB entry 1hw2 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
The FadR.DNA complex. Transcriptional control of fatty acid metabolism in Escherichia coli.
Xu Y, Heath RJ, Li Z, Rock CO, White SW
J Biol Chem 276 17373-9 (2001)
PMID: 11279025

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-113918 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Fatty acid metabolism regulator protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 239 amino acids
Theoretical weight: 27 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A8V6 (Residues: 1-239; Coverage: 100%)
Gene names: JW1176, b1187, fadR, oleR, thdB
Sequence domains:
Structure domains:
5'-D(*CP*GP*AP*TP*CP*TP*GP*GP*TP*CP*CP*GP*AP*CP*CP*AP*GP*AP*TP*GP*CP*T)-3' Chain: D
Molecule details ›
Chain: D
Length: 22 nucleotides
Theoretical weight: 6.73 KDa
Source organism: Escherichia coli
Expression system: Not provided
5'-D(*G*CP*AP*TP*CP*TP*GP*GP*TP*CP*GP*GP*AP*CP*CP*AP*GP*AP*TP*CP*GP*A)-3' Chain: E
Molecule details ›
Chain: E
Length: 22 nucleotides
Theoretical weight: 6.78 KDa
Source organism: Escherichia coli
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P212121
Unit cell:
a: 83.733Å b: 110.148Å c: 130.281Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.235 0.235 0.278
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

12 review citations

Diversity of microbial sialic acid metabolism.
Vimr et al. (2004)
11 more

12 mentions without citation

Transcription factor-based biosensors enlightened by the analyte.
Fernandez-López et al. (2015)
11 more