1huz

X-ray diffraction
2.6Å resolution

CRYSTAL STRUCTURE OF DNA POLYMERASE COMPLEXED WITH DNA AND CR-PCP

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
DNA polymerase beta Chains: A, B
Molecule details ›
Chains: A, B
Length: 335 amino acids
Theoretical weight: 38.39 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P06766 (Residues: 1-335; Coverage: 100%)
Gene name: Polb
Sequence domains:
Structure domains:
5'-D(*AP*AP*TP*AP*GP*GP*CP*GP*TP*CP*G)-3' Chains: C, T
Molecule details ›
Chains: C, T
Length: 11 nucleotides
Theoretical weight: 3.4 KDa
Source organism: Rattus norvegicus
Expression system: Not provided
5'-D(P*CP*GP*AP*CP*GP*CP*CP*T)-3' Chains: D, P
Molecule details ›
Chains: D, P
Length: 8 nucleotides
Theoretical weight: 2.39 KDa
Source organism: Rattus norvegicus
Expression system: Not provided

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: P21
Unit cell:
a: 105.78Å b: 56.134Å c: 85.414Å
α: 90° β: 106.94° γ: 90°
R-values:
R R work R free
0.224 0.224 0.286
Expression systems:
  • Escherichia coli
  • Not provided