1huj

X-ray diffraction
2.1Å resolution

REFINED STRUCTURE OF YEAST INORGANIC PYROPHOSPHATASE AND ITS K61R MUTANT

Released:
Source organism: Saccharomyces cerevisiae
Entry authors: Swaminathan K, Cooperman BS, Lahti R, Voet D

Function and Biology Details

Reaction catalysed:
Diphosphate + H(2)O = 2 phosphate
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Inorganic pyrophosphatase Chains: A, B
Molecule details ›
Chains: A, B
Length: 281 amino acids
Theoretical weight: 31.81 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P00817 (Residues: 2-282; Coverage: 98%)
Gene names: IPP1, PPA, PPA1, YBR011C, YBR0202
Sequence domains: Inorganic pyrophosphatase
Structure domains: Inorganic pyrophosphatase

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P21
Unit cell:
a: 69.05Å b: 92.52Å c: 51.58Å
α: 90° β: 99.47° γ: 90°
R-values:
R R work R free
0.209 0.209 0.272
Expression system: Escherichia coli