› 1hu8

X-ray diffraction
2.7Å resolution

CRYSTAL STRUCTURE OF THE MOUSE P53 CORE DNA-BINDING DOMAIN AT 2.7A RESOLUTION

Released:
DOI: 10.2210/pdb1hu8/pdb
PDB 1hu8 coloured by chain and viewed from the front.
PDB 1hu8 coloured by chain and viewed from the side.
PDB 1hu8 coloured by chain and viewed from the top.
Source organism: Mus musculus
Primary publication:
Crystal structure of the mouse p53 core DNA-binding domain at 2.7 A resolution.
Zhao K, Chai X, Johnston K, Clements A, Marmorstein R
J. Biol. Chem. 276 12120-7 (2001)
PMID: 11152481

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cellular tumor antigen p53 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 186 amino acids
Theoretical weight: 20.93 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P02340 (Residues: 99-284; Coverage: 48%)
Gene names: P53, Tp53, Trp53
Sequence domains: P53 DNA-binding domain
Structure domains: Immunoglobulin-like

Ligands and Environments

1 bound ligand:
Ligand ZN 3 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE A1
Spacegroup: C2221
Unit cell:
a: 73.676Å b: 119.992Å c: 184.238Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.265 0.239 0.299
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

13 review citations

Regulation of p53 by E3s.
Pan et al. (2021)
12 more

4 mentions without citation

The structure of p53 tumour suppressor protein reveals the basis for its functional plasticity.
Okorokov et al. (2006)
3 more