› 1hlv

X-ray diffraction
2.5Å resolution

CRYSTAL STRUCTURE OF CENP-B(1-129) COMPLEXED WITH THE CENP-B BOX DNA

Released:
DOI: 10.2210/pdb1hlv/pdb
PDB entry 1hlv coloured by chain, front view.
PDB entry 1hlv coloured by chain, side view.
PDB entry 1hlv coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of the CENP-B protein-DNA complex: the DNA-binding domains of CENP-B induce kinks in the CENP-B box DNA.
Tanaka Y, Nureki O, Kurumizaka H, Fukai S, Kawaguchi S, Ikuta M, Iwahara J, Okazaki T, Yokoyama S
EMBO J 20 6612-8 (2001)
PMID: 11726497

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-113903 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Major centromere autoantigen B Chain: A
Molecule details ›
Chain: A
Length: 131 amino acids
Theoretical weight: 15.17 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P07199 (Residues: 1-129; Coverage: 22%)
Gene name: CENPB
Sequence domains:
Structure domains: Homeodomain-like
CENP-B BOX DNA Chain: B
Molecule details ›
Chain: B
Length: 21 nucleotides
Theoretical weight: 6.49 KDa
Source organism: Homo sapiens
Expression system: Not provided
CENP-B BOX DNA Chain: C
Molecule details ›
Chain: C
Length: 21 nucleotides
Theoretical weight: 6.39 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44B2
Spacegroup: P3112
Unit cell:
a: 83.374Å b: 83.374Å c: 139.021Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.222 0.222 0.26
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

12 review citations

The many faces of the helix-turn-helix domain: transcription regulation and beyond.
Aravind et al. (2005)
11 more

15 mentions without citation

Protein flexibility in the light of structural alphabets.
Craveur et al. (2015)
14 more