› 1hld

X-ray diffraction
2.1Å resolution

STRUCTURES OF HORSE LIVER ALCOHOL DEHYDROGENASE COMPLEXED WITH NAD+ AND SUBSTITUTED BENZYL ALCOHOLS

Released:
DOI: 10.2210/pdb1hld/pdb
PDB entry 1hld coloured by chain, front view.
PDB entry 1hld coloured by chain, side view.
PDB entry 1hld coloured by chain, top view.
Source organism: Equus caballus
Primary publication:
Structures of horse liver alcohol dehydrogenase complexed with NAD+ and substituted benzyl alcohols.
Ramaswamy S, Eklund H, Plapp BV
Biochemistry 33 5230-7 (1994)
PMID: 8172897

Function and Biology Details

Reaction catalysed: 
A primary alcohol + NAD(+) = an aldehyde + NADH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132405 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alcohol dehydrogenase E chain Chains: A, B
Molecule details ›
Chains: A, B
Length: 374 amino acids
Theoretical weight: 39.85 KDa
Source organism: Equus caballus
Expression system: Not provided
UniProt:
  • Canonical: P00327 (Residues: 2-375; Coverage: 100%)
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand NAD 2 x NAD
3 bound ligands:
Ligand ZN 4 x ZN
Ligand PFB 2 x PFB
Ligand BRB 2 x BRB
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P21
Unit cell:
a: 51.1Å b: 180.7Å c: 44.3Å
α: 90° β: 108° γ: 90°
R-values:
R R work R free
0.183 0.183 not available
Expression system: Not provided

Quick links

Citations

9 review citations

Relating protein motion to catalysis.
Hammes-Schiffer et al. (2006)
8 more

11 mentions without citation

Medium- and short-chain dehydrogenase/reductase gene and protein families : the SDR superfamily: functional and structural diversity within a family of metabolic and regulatory enzymes.
Kavanagh et al. (2008)
10 more