X-ray diffraction
2.55Å resolution

Structure of two fungal beta-1,4-galactanases: searching for the basis for temperature and pH optimum.


Function and Biology Details

Reaction catalysed:
The enzyme specifically hydrolyzes (1->4)-beta-D-galactosidic linkages in type I arabinogalactans.
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Arabinogalactan endo-beta-1,4-galactanase Chain: A
Molecule details ›
Chain: A
Length: 332 amino acids
Theoretical weight: 37.69 KDa
Source organism: Humicola insolens
Expression system: Aspergillus oryzae
  • Canonical: P83691 (Residues: 1-332; Coverage: 100%)
Sequence domains: Glycosyl hydrolase family 53
Structure domains: Glycosidases

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MAX II BEAMLINE I711
Spacegroup: P212121
Unit cell:
a: 45.254Å b: 68.955Å c: 135.16Å
α: 90° β: 90° γ: 90°
R R work R free
0.199 0.199 0.247
Expression system: Aspergillus oryzae