1hjf

X-ray diffraction
1.6Å resolution

Alteration of the co-substrate selectivity of deacetoxycephalosporin C synthase: The role of arginine-258

Released:

Function and Biology Details

Reaction catalysed:
Penicillin N + 2-oxoglutarate + O(2) = deacetoxycephalosporin C + succinate + CO(2) + H(2)O
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Deacetoxycephalosporin C synthase Chain: A
Molecule details ›
Chain: A
Length: 311 amino acids
Theoretical weight: 34.56 KDa
Source organism: Streptomyces clavuligerus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P18548 (Residues: 1-311; Coverage: 100%)
Gene name: cefE
Sequence domains:
Structure domains: B-lactam Antibiotic, Isopenicillin N Synthase; Chain

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX9.6
Spacegroup: R3
Unit cell:
a: 106.933Å b: 106.933Å c: 70.819Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.241 0.241 0.269
Expression system: Escherichia coli BL21(DE3)