1hg8

X-ray diffraction
1.73Å resolution

Endopolygalacturonase from the phytopathogenic fungus Fusarium moniliforme

Released:

Function and Biology Details

Reaction catalysed:
(1,4-alpha-D-galacturonosyl)(n+m) + H(2)O = (1,4-alpha-D-galacturonosyl)(n) + (1,4-alpha-D-galacturonosyl)(m)
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Polygalacturonase Chain: A
Molecule details ›
Chain: A
Length: 349 amino acids
Theoretical weight: 36.22 KDa
Source organism: Fusarium verticillioides
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: Q07181 (Residues: 25-373; Coverage: 100%)
Gene name: PGA
Sequence domains: Glycosyl hydrolases family 28
Structure domains: Single-stranded right-handed beta-helix, Pectin lyase-like

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ELETTRA BEAMLINE 5.2R
Spacegroup: P212121
Unit cell:
a: 58.26Å b: 61.59Å c: 98.86Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.173 0.173 0.203
Expression system: Saccharomyces cerevisiae