› 1hg2

X-ray diffraction
2Å resolution

CALM-N N-terminal domain of clathrin assembly lymphoid myeloid leukaemia protein, Inositol(4,5)P2 complex

Released:
DOI: 10.2210/pdb1hg2/pdb
PDB entry 1hg2 coloured by chain, front view.
PDB entry 1hg2 coloured by chain, side view.
PDB entry 1hg2 coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
Simultaneous binding of PtdIns(4,5)P2 and clathrin by AP180 in the nucleation of clathrin lattices on membranes.
Ford MG, Pearse BM, Higgins MK, Vallis Y, Owen DJ, Gibson A, Hopkins CR, Evans PR, McMahon HT
Science 291 1051-5 (2001)
PMID: 11161218

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129534 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phosphatidylinositol-binding clathrin assembly protein Chain: A
Molecule details ›
Chain: A
Length: 289 amino acids
Theoretical weight: 32.87 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: O55012 (Residues: 1-289; Coverage: 45%)
Gene names: Calm, Picalm
Sequence domains: ANTH domain
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand IP2 1 x IP2
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX9.6
Spacegroup: P41212
Unit cell:
a: 77.859Å b: 77.859Å c: 122.333Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.198 0.197 0.217
Expression system: Escherichia coli BL21

Quick links

Citations

136 review citations

Regulated portals of entry into the cell.
Conner et al. (2003)
135 more

3 mentions without citation

Molecular structure, function, and dynamics of clathrin-mediated membrane traffic.
Kirchhausen et al. (2014)
2 more