Structure analysis

Ternary Complex of SAP-1 and SRF with specific SRE DNA

X-ray diffraction
3.15Å resolution
Source organism: Homo sapiens
Assembly composition:
hetero pentamer (preferred)
Entry contents: 2 distinct polypeptide molecules
2 distinct DNA molecules

Assemblies

Assembly 1 (preferred)
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Multimeric state: hetero pentamer
Accessible surface area: 19800 Å2
Buried surface area: 15100 Å2
Dissociation area: 2,000 Å2
Dissociation energy (ΔGdiss): 27 kcal/mol
Dissociation entropy (TΔSdiss): 13 kcal/mol
Interface energy (ΔGint): -116 kcal/mol
Symmetry number: 1
Assembly 2
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Multimeric state: hetero pentamer
Accessible surface area: 19700 Å2
Buried surface area: 14600 Å2
Dissociation area: 1,900 Å2
Dissociation energy (ΔGdiss): 23 kcal/mol
Dissociation entropy (TΔSdiss): 13 kcal/mol
Interface energy (ΔGint): -105 kcal/mol
Symmetry number: 1

Macromolecules

Chains: A, B, D, E
Length: 92 amino acids
Theoretical weight: 10.3 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P11831 (Residues: 132-223; Coverage: 18%)
Gene name: SRF
Pfam: SRF-type transcription factor (DNA-binding and dimerisation domain)
InterPro:
CATH: Transcription factor, MADS-box
SCOP: SRF-like

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Chains: G, H
Length: 157 amino acids
Theoretical weight: 17.97 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P28324 (Residues: 2-156; Coverage: 36%)
Gene names: ELK4, SAP1
Pfam: Ets-domain
InterPro:
CATH: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain
SCOP: ets domain

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Name: 5'-D(*GP*AP*TP*GP*GP*CP*CP*TP*AP*AP*TP*TP*AP* GP*GP*AP*CP*TP*TP*CP*CP*GP*GP*TP*G)-3'
Representative chains: C, F
Length: 26 nucleotides
Theoretical weight: 8.03 KDa

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Name: 5'-D(*CP*AP*CP*AP*CP*CP*GP*GP*AP*AP*GP*TP*CP* CP*TP*AP*AP*TP*TP*AP*GP*GP*CP*CP*AP*T)-3'
Representative chains: W, X
Length: 26 nucleotides
Theoretical weight: 7.94 KDa

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