X-ray diffraction
2Å resolution

nuclear Cap Binding Complex

Source organism: Homo sapiens
Primary publication:
Crystal structure of the human nuclear cap binding complex.
Mol. Cell 8 383-96 (2001)
PMID: 11545740

Function and Biology Details

Structure analysis Details

Assembly composition:
Non-polymer only dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Nuclear cap-binding protein subunit 1 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 757 amino acids
Theoretical weight: 87.94 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
  • Canonical: Q09161 (Residues: 20-790; Coverage: 96%)
Gene names: CBP80, NCBP, NCBP1
Sequence domains:
Structure domains: Serine Threonine Protein Phosphatase 5, Tetratricopeptide repeat
Nuclear cap-binding protein subunit 2 Chains: X, Y, Z
Molecule details ›
Chains: X, Y, Z
Length: 98 amino acids
Theoretical weight: 11.5 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
  • Canonical: P52298 (Residues: 22-120; Coverage: 63%)
Gene names: CBP20, NCBP2, PIG55
Sequence domains: RNA recognition motif. (a.k.a. RRM, RBD, or RNP domain)
Structure domains: Alpha-Beta Plaits

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P212121
Unit cell:
a: 75.527Å b: 161.48Å c: 303.291Å
α: 90° β: 90° γ: 90°
R R work R free
0.203 0.203 0.234
Expression systems:
  • Trichoplusia ni
  • Escherichia coli BL21