1h1l

X-ray diffraction
1.9Å resolution

NITROGENASE MO-FE PROTEIN FROM KLEBSIELLA PNEUMONIAE, NIFV MUTANT

Released:

Function and Biology Details

Reaction catalysed:
8 reduced ferredoxin + 8 H(+) + N(2) + 16 ATP + 16 H(2)O = 8 oxidized ferredoxin + H(2) + 2 NH(3) + 16 ADP + 16 phosphate
Biochemical function:
Biological process:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Nitrogenase molybdenum-iron protein alpha chain Chains: A, C
Molecule details ›
Chains: A, C
Length: 481 amino acids
Theoretical weight: 53.92 KDa
Source organism: Klebsiella pneumoniae
UniProt:
  • Canonical: P00466 (Residues: 3-483; Coverage: 100%)
Gene name: nifD
Sequence domains: Nitrogenase component 1 type Oxidoreductase
Structure domains: Nitrogenase molybdenum iron protein domain
Nitrogenase molybdenum-iron protein beta chain Chains: B, D
Molecule details ›
Chains: B, D
Length: 519 amino acids
Theoretical weight: 58.34 KDa
Source organism: Klebsiella pneumoniae
UniProt:
  • Canonical: P09772 (Residues: 2-520; Coverage: 100%)
Gene name: nifK
Sequence domains: Nitrogenase component 1 type Oxidoreductase
Structure domains:

Ligands and Environments

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: C2
Unit cell:
a: 204.06Å b: 75.06Å c: 164.26Å
α: 90° β: 123.95° γ: 90°
R-values:
R R work R free
0.176 0.176 0.236