1h16

X-ray diffraction
1.53Å resolution

Pyruvate Formate-Lyase (E.coli) in complex with Pyruvate and CoA

Released:

Function and Biology Details

Reaction catalysed:
Acetyl-CoA + formate = CoA + pyruvate
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Formate acetyltransferase 1 Chain: A
Molecule details ›
Chain: A
Length: 759 amino acids
Theoretical weight: 85.33 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P09373 (Residues: 2-760; Coverage: 100%)
Gene names: JW0886, b0903, pfl, pflB
Sequence domains: Glycine radical
Structure domains: Anaerobic Ribonucleotide-triphosphate Reductase Large Chain

Ligands and Environments


Cofactor: Ligand COA 1 x COA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X13
Spacegroup: C2221
Unit cell:
a: 54.9Å b: 153.05Å c: 205.95Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.145 0.145 0.163