› 1gzh

X-ray diffraction
2.6Å resolution

Crystal structure of the BRCT domains of human 53BP1 bound to the p53 tumor supressor

Released:
DOI: 10.2210/pdb1gzh/pdb
PDB 1gzh coloured by chain and viewed from the front.
PDB 1gzh coloured by chain and viewed from the side.
PDB 1gzh coloured by chain and viewed from the top.
Source organism: Homo sapiens
Primary publication:
Crystal structure of human 53BP1 BRCT domains bound to p53 tumour suppressor.
Derbyshire DJ, Basu BP, Serpell LC, Joo WS, Date T, Iwabuchi K, Doherty AJ
EMBO J. 21 3863-72 (2002)
PMID: 12110597

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Cellular tumor antigen p53 Chain: A
Molecule details ›
Chain: A
Length: 198 amino acids
Theoretical weight: 22.38 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P04637 (Residues: 95-292; Coverage: 50%)
Gene names: P53, TP53
Sequence domains: P53 DNA-binding domain
Structure domains: Immunoglobulin-like
TP53-binding protein 1 Chains: B, D
Molecule details ›
Chains: B, D
Length: 249 amino acids
Theoretical weight: 28.18 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q12888 (Residues: 1724-1972; Coverage: 13%)
Gene name: TP53BP1
Sequence domains: BRCA1 C Terminus (BRCT) domain
Structure domains: BRCT domain
Cellular tumor antigen p53 Chain: C
Molecule details ›
Chain: C
Length: 198 amino acids
Theoretical weight: 22.36 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P04637 (Residues: 95-292; Coverage: 50%)
Gene names: P53, TP53
Sequence domains: P53 DNA-binding domain
Structure domains: Immunoglobulin-like

Ligands and Environments

2 bound ligands:
Ligand ZN 2 x ZN
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P212121
Unit cell:
a: 71.52Å b: 94.57Å c: 136.2Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.238 0.238 0.288
Expression system: Escherichia coli

Quick links

Citations

17 review citations

53BP1: a DSB escort.
Mirman et al. (2020)
16 more

17 mentions without citation

Classification of intrinsically disordered regions and proteins.
van der Lee et al. (2014)
16 more