› 1gyx

X-ray diffraction
1.35Å resolution

The Crystal Structure of YdcE, a 4-Oxalocrotonate Tautomerase Homologue from Escherichia coli, Confirms the Structural Basis for Oligomer Diversity

Released:
DOI: 10.2210/pdb1gyx/pdb
PDB entry 1gyx coloured by chain, front view.
PDB entry 1gyx coloured by chain, side view.
PDB entry 1gyx coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
The crystal structure of YdcE, a 4-oxalocrotonate tautomerase homologue from Escherichia coli, confirms the structural basis for oligomer diversity.
Almrud JJ, Kern AD, Wang SC, Czerwinski RM, Johnson WH, Murzin AG, Hackert ML, Whitman CP
Biochemistry 41 12010-24 (2002)
PMID: 12356301

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152182 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tautomerase PptA Chains: A, B
Molecule details ›
Chains: A, B
Length: 76 amino acids
Theoretical weight: 8.55 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P31992 (Residues: 2-77; Coverage: 99%)
Gene names: JW1456, b1461, pptA, ydcE
Sequence domains: Tautomerase enzyme
Structure domains: Macrophage Migration Inhibitory Factor

Ligands and Environments

2 bound ligands:
Ligand BEZ 1 x BEZ
Ligand EPE 1 x EPE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P212121
Unit cell:
a: 44.566Å b: 46.525Å c: 75.724Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.142 0.142 0.164
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

The chemical versatility of the beta-alpha-beta fold: catalytic promiscuity and divergent evolution in the tautomerase superfamily.
Poelarends et al. (2008)
2 more

4 mentions without citation

Calculating ensemble averaged descriptions of protein rigidity without sampling.
González et al. (2012)
3 more