Solution NMR

Non-cognate protein-protein interactions: the NMR structure of the colicin E8 inhibitor protein Im8 and its interaction with the DNase domain of colicin E9

Source organism: Escherichia coli
Entry authors: Le Duff CS, Videler H, Boetzel R, Czisch M, James R, Kleanthous C, Moore GR

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Colicin-E8 immunity protein Chain: A
Molecule details ›
Chain: A
Length: 85 amino acids
Theoretical weight: 9.63 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
  • Canonical: P09881 (Residues: 1-85; Coverage: 100%)
Gene names: ceiE8, imm
Sequence domains: Colicin immunity protein / pyocin immunity protein
Structure domains: Colicin E immunity protein

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: torsion angle dynamics
Expression system: Escherichia coli BL21(DE3)