› 1gui

X-ray diffraction
1.9Å resolution

CBM4 structure and function

Released:
DOI: 10.2210/pdb1gui/pdb
PDB entry 1gui coloured by chain, front view.
PDB entry 1gui coloured by chain, side view.
PDB entry 1gui coloured by chain, top view.
Source organism: Thermotoga maritima
Primary publication:
Differential oligosaccharide recognition by evolutionarily-related beta-1,4 and beta-1,3 glucan-binding modules.
Boraston AB, Nurizzo D, Notenboom V, Ducros V, Rose DR, Kilburn DG, Davies GJ
J Mol Biol 319 1143-56 (2002)
PMID: 12079353

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-194425 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
GH16 domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 155 amino acids
Theoretical weight: 17.61 KDa
Source organism: Thermotoga maritima
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q9WXN1 (Residues: 488-642; Coverage: 24%)
Gene name: TM_0024
Sequence domains: Carbohydrate binding domain
Structure domains: Galactose-binding domain-like

Ligands and Environments

Carbohydrate polymer : NEW Components: BGC
Carbohydrate polymer
2 bound ligands:
Ligand CA 1 x CA
Ligand GOL 5 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P3221
Unit cell:
a: 105.117Å b: 105.117Å c: 49.636Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.173 0.172 0.197
Expression system: Escherichia coli BL21

Quick links

Citations

14 review citations

Role of receptors in Bacillus thuringiensis crystal toxin activity.
Pigott et al. (2007)
13 more

20 mentions without citation

Carbohydrate-binding modules: fine-tuning polysaccharide recognition.
Boraston et al. (2004)
19 more