› 1gu0

X-ray diffraction
2Å resolution

CRYSTAL STRUCTURE OF TYPE II DEHYDROQUINASE FROM STREPTOMYCES COELICOLOR

Released:
DOI: 10.2210/pdb1gu0/pdb
PDB entry 1gu0 coloured by chain, front view.
PDB entry 1gu0 coloured by chain, side view.
PDB entry 1gu0 coloured by chain, top view.
Source organism: Streptomyces coelicolor
Primary publication:
The structure and mechanism of the type II dehydroquinase from Streptomyces coelicolor.
Roszak AW, Robinson DA, Krell T, Hunter IS, Fredrickson M, Abell C, Coggins JR, Lapthorn AJ
Structure 10 493-503 (2002)
PMID: 11937054

Function and Biology Details

Reaction catalysed: 
3-dehydroquinate = 3-dehydroshikimate + H(2)O
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dodecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147381 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
3-dehydroquinate dehydratase Chains: A, B, C, D, E, F, G, H, I, J, K, L
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L
Length: 156 amino acids
Theoretical weight: 16.57 KDa
Source organism: Streptomyces coelicolor
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P15474 (Residues: 2-157; Coverage: 99%)
Gene names: SCC54.21c, SCO1961, aroQ
Sequence domains: Dehydroquinase class II
Structure domains: Dehydroquinase, class II

Ligands and Environments

1 bound ligand:
Ligand TRS 4 x TRS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX9.6
Spacegroup: P212121
Unit cell:
a: 116.2Å b: 138.4Å c: 141.2Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.204 0.2 0.242
Expression system: Escherichia coli BL21

Quick links

Citations

3 review citations

A Three-Ring Circus: Metabolism of the Three Proteogenic Aromatic Amino Acids and Their Role in the Health of Plants and Animals.
Parthasarathy et al. (2018)
2 more