› 1gro

X-ray diffraction
2.5Å resolution

REGULATORY AND CATALYTIC MECHANISMS IN ESCHERICHIA COLI ISOCITRATE DEHYDROGENASE: MULTIPLE ROLES FOR N115

Released:
DOI: 10.2210/pdb1gro/pdb
PDB entry 1gro coloured by chain, front view.
PDB entry 1gro coloured by chain, side view.
PDB entry 1gro coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Second-site suppression of regulatory phosphorylation in Escherichia coli isocitrate dehydrogenase.
Chen R, Grobler JA, Hurley JH, Dean AM
Protein Sci 5 287-95 (1996)
PMID: 8745407

Function and Biology Details

Reaction catalysed: 
(1a) isocitrate + NADP(+) = 2-oxalosuccinate + NADPH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140025 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Isocitrate dehydrogenase [NADP] Chain: A
Molecule details ›
Chain: A
Length: 416 amino acids
Theoretical weight: 45.84 KDa
Source organism: Escherichia coli
Expression system: Not provided
UniProt:
  • Canonical: P08200 (Residues: 1-416; Coverage: 100%)
Gene names: JW1122, b1136, icd, icdA, icdE
Sequence domains: Isocitrate/isopropylmalate dehydrogenase
Structure domains: Isopropylmalate Dehydrogenase

Ligands and Environments

2 bound ligands:
Ligand MG 1 x MG
Ligand ICT 1 x ICT
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P43212
Unit cell:
a: 105.1Å b: 105.1Å c: 150.6Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.195 0.195 not available
Expression system: Not provided

Quick links

Citations

2 review citations

Regulation of acetate metabolism by protein phosphorylation in enteric bacteria.
Cozzone AJ. (1998)
1 more

4 mentions without citation

The sweet side of the pathogenic Neisseria: the role of glycan interactions in colonisation and disease.
Mubaiwa et al. (2017)
3 more