1grn

X-ray diffraction
2.1Å resolution

CRYSTAL STRUCTURE OF THE CDC42/CDC42GAP/ALF3 COMPLEX.

Released:
Source organism: Homo sapiens

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Cell division control protein 42 homolog Chain: A
Molecule details ›
Chain: A
Length: 191 amino acids
Theoretical weight: 21.28 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P60953 (Residues: 1-191; Coverage: 100%)
Gene name: CDC42
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases
Rho GTPase-activating protein 1 Chain: B
Molecule details ›
Chain: B
Length: 203 amino acids
Theoretical weight: 23.04 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q07960 (Residues: 237-439; Coverage: 46%)
Gene names: ARHGAP1, CDC42GAP, RHOGAP1
Sequence domains: RhoGAP domain
Structure domains: Rho GTPase activation protein

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE F2
Spacegroup: P212121
Unit cell:
a: 52.4Å b: 69Å c: 129.6Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.211 0.211 0.259
Expression system: Escherichia coli