› 1gqc

X-ray diffraction
2.6Å resolution

THE STRUCTURE OF CMP:2-KETO-3-DEOXY-MANNO-OCTONIC ACID SYNTHETASE COMPLEXED WITH CMP-Kdo at 100K

Released:
DOI: 10.2210/pdb1gqc/pdb
PDB entry 1gqc coloured by chain, front view.
PDB entry 1gqc coloured by chain, side view.
PDB entry 1gqc coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Catalytic mechanism of CMP:2-keto-3-deoxy-manno-octonic acid synthetase as derived from complexes with reaction educt and product.
Jelakovic S, Schulz GE
Biochemistry 41 1174-81 (2002)
PMID: 11802716

Function and Biology Details

Reaction catalysed: 
CTP + 3-deoxy-D-manno-octulosonate = diphosphate + CMP-3-deoxy-D-manno-octulosonate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154566 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
3-deoxy-manno-octulosonate cytidylyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 245 amino acids
Theoretical weight: 27.06 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P42216 (Residues: 2-246; Coverage: 100%)
Gene name: kpsU
Sequence domains: Cytidylyltransferase
Structure domains: Spore Coat Polysaccharide Biosynthesis Protein SpsA; Chain A

Ligands and Environments

3 bound ligands:
Ligand MG 2 x MG
Ligand CMK 1 x CMK
Ligand C5P 1 x C5P
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P21
Unit cell:
a: 45.944Å b: 130.996Å c: 48.148Å
α: 90° β: 102.31° γ: 90°
R-values:
R R work R free
0.178 0.178 0.24
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Kdo: a critical monosaccharide for bacteria viability.
Cipolla et al. (2010)
1 more

4 mentions without citation

Structure-based mechanism of CMP-2-keto-3-deoxymanno-octulonic acid synthetase: convergent evolution of a sugar-activating enzyme with DNA/RNA polymerases.
Heyes et al. (2009)
3 more