1gq9

X-ray diffraction
2.6Å resolution

THE STRUCTURE OF CMP:2-KETO-3-DEOXY-MANNO-OCTONIC ACID SYNTHETASE COMPLEXED WITH CTP at 100K

Released:

Function and Biology Details

Reaction catalysed:
CTP + 3-deoxy-D-manno-octulosonate = diphosphate + CMP-3-deoxy-D-manno-octulosonate
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
3-deoxy-manno-octulosonate cytidylyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 245 amino acids
Theoretical weight: 27.06 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P42216 (Residues: 2-246; Coverage: 100%)
Gene name: kpsU
Sequence domains: Cytidylyltransferase
Structure domains: Spore Coat Polysaccharide Biosynthesis Protein SpsA; Chain A

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P21
Unit cell:
a: 45.671Å b: 132.349Å c: 47.908Å
α: 90° β: 102.37° γ: 90°
R-values:
R R work R free
0.233 0.233 0.289
Expression system: Escherichia coli