1gp6

X-ray diffraction
1.75Å resolution

Anthocyanidin synthase from Arabidopsis thaliana complexed with trans-dihydroquercetin (with 30 min exposure to O2)

Released:

Function and Biology Details

Reaction catalysed:
(1a) a (2R,3S,4S)-leucoanthocyanidin + 2-oxoglutarate + O(2) = a (4S)- 2,3-dehydroflavan-3,4-diol + succinate + CO(2) + H(2)O
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Leucoanthocyanidin dioxygenase Chain: A
Molecule details ›
Chain: A
Length: 356 amino acids
Theoretical weight: 40.45 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q96323 (Residues: 1-356; Coverage: 100%)
Gene names: At4g22880, F7H19.60, LDOX
Sequence domains:
Structure domains: B-lactam Antibiotic, Isopenicillin N Synthase; Chain

Ligands and Environments


No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX9.6
Spacegroup: P212121
Unit cell:
a: 61.258Å b: 72.385Å c: 87.054Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.194 0.194 0.222
Expression system: Escherichia coli BL21(DE3)