PDBe 1go4

X-ray diffraction
2.05Å resolution

Crystal structure of Mad1-Mad2 reveals a conserved Mad2 binding motif in Mad1 and Cdc20.

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Mitotic spindle assembly checkpoint protein MAD2A Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 205 amino acids
Theoretical weight: 23.45 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q13257 (Residues: 1-205; Coverage: 100%)
  • Best match: Q13257-2 (Residues: 1-73)
Gene names: MAD2, MAD2L1
Sequence domains: HORMA domain
Structure domains: HORMA domain
Mitotic spindle assembly checkpoint protein MAD1 Chains: E, F, G, H
Molecule details ›
Chains: E, F, G, H
Length: 100 amino acids
Theoretical weight: 11.69 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9Y6D9 (Residues: 485-584; Coverage: 14%)
Gene names: MAD1, MAD1L1, TXBP181

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF
Spacegroup: P21
Unit cell:
a: 111.037Å b: 63.023Å c: 139.511Å
α: 90° β: 111.65° γ: 90°
R-values:
R R work R free
0.24 0.24 0.268
Expression system: Escherichia coli