› 1gmo

X-ray diffraction
3Å resolution

CRYSTAL STRUCTURES OF NK1-HEPARIN COMPLEXES REVEAL THE BASIS FOR NK1 ACTIVITY AND ENABLE ENGINEERING OF POTENT AGONISTS OF THE MET RECEPTOR

Released:
DOI: 10.2210/pdb1gmo/pdb
PDB 1gmo coloured by chain and viewed from the front.
PDB 1gmo coloured by chain and viewed from the side.
PDB 1gmo coloured by chain and viewed from the top.
Source organism: Homo sapiens
Primary publication:
Crystal structures of NK1-heparin complexes reveal the basis for NK1 activity and enable engineering of potent agonists of the MET receptor.
Lietha D, Chirgadze DY, Mulloy B, Blundell TL, Gherardi E
EMBO J. 20 5543-55 (2001)
PMID: 11597998

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (6 distinct):
Hepatocyte growth factor alpha chain Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 183 amino acids
Theoretical weight: 21.17 KDa
Source organism: Homo sapiens
Expression system: Komagataella phaffii GS115
UniProt:
  • Canonical: P14210 (Residues: 28-210; Coverage: 26%)
Gene names: HGF, HPTA
Sequence domains:
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: IDS, SGN
Ligand IDS 3 x IDS
Ligand SGN 3 x SGN
Carbohydrate polymer : NEW Components: IDS, SGN
Ligand IDS 4 x IDS
Ligand SGN 3 x SGN
Carbohydrate polymer : NEW Components: IDS, SGN
Ligand IDS 4 x IDS
Ligand SGN 4 x SGN
Carbohydrate polymer : NEW Components: IDS, SGN
Ligand IDS 4 x IDS
Ligand SGN 5 x SGN
Carbohydrate polymer : NEW Components: IDS, SGN
Ligand IDS 6 x IDS
Ligand SGN 6 x SGN
2 bound ligands:
Ligand SO4 8 x SO4
Ligand EPE 8 x EPE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX14.2
Spacegroup: P21212
Unit cell:
a: 179.7Å b: 174.1Å c: 59.9Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.254 0.254 0.295
Expression system: Komagataella phaffii GS115

Quick links

Citations

17 review citations

The development of small molecule angiotensin IV analogs to treat Alzheimer's and Parkinson's diseases.
Wright et al. (2015)
16 more

7 mentions without citation

Achieving high signal-to-noise in cell regulatory systems: Spatial organization of multiprotein transmembrane assemblies of FGFR and MET receptors.
Blaszczyk et al. (2015)
6 more