› 1gll

X-ray diffraction
3Å resolution

ESCHERICHIA COLI GLYCEROL KINASE MUTANT WITH BOUND ATP ANALOG SHOWING SUBSTANTIAL DOMAIN MOTION

Released:
DOI: 10.2210/pdb1gll/pdb
PDB entry 1gll coloured by chain, front view.
PDB entry 1gll coloured by chain, side view.
PDB entry 1gll coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Crystal structures of Escherichia coli glycerol kinase variant S58-->W in complex with nonhydrolyzable ATP analogues reveal a putative active conformation of the enzyme as a result of domain motion.
Bystrom CE, Pettigrew DW, Branchaud BP, O'Brien P, Remington SJ
Biochemistry 38 3508-18 (1999)
PMID: 10090737

Function and Biology Details

Reaction catalysed: 
ATP + glycerol = ADP + sn-glycerol 3-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141309 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glycerol kinase Chains: O, Y
Molecule details ›
Chains: O, Y
Length: 501 amino acids
Theoretical weight: 56.26 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A6F3 (Residues: 2-502; Coverage: 100%)
Gene names: JW3897, b3926, glpK
Sequence domains:
Structure domains: Nucleotidyltransferase; domain 5

Ligands and Environments

3 bound ligands:
Ligand MG 2 x MG
Ligand ACP 2 x ACP
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: C2221
Unit cell:
a: 99.766Å b: 201.148Å c: 114.34Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.176 0.176 not available
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Dynamic dissociating homo-oligomers and the control of protein function.
Selwood et al. (2012)
1 more

2 mentions without citation

The FGGY carbohydrate kinase family: insights into the evolution of functional specificities.
Zhang et al. (2011)
1 more