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Refined structure of the complex between guanylate kinase and its substrate GMP at 2.0 A resolution.

Stehle T, Schulz GE
J Mol Biol 224 1127-41 (1992)
Cited: 84 times
EuropePMC logo PMID: 1314905

Abstract

The crystal structure of guanylate kinase from Saccharomyces cerevisiae complexed with its substrate GMP has been refined at a resolution of 2.0 A. The final crystallographic R-factor is 17.3% in the resolution range 7.0 A to 2.0 A for all reflections of the 100% complete data set. The final model has standard geometry with root-mean-square deviations of 0.016 A in bond lengths and 3.0 in bond angles. It consists of all 186 amino acid residues, the N-terminal acetyl group, the substrate GMP, one sulfate ion and 174 water molecules. Guanylate kinase is structurally related to adenylate kinases and G-proteins with respect to its central beta-sheet with connecting helices and the giant anion hole that binds nucleoside triphosphates. These nucleotides are ATP and GTP for the kinases and GTP for the G-proteins. The chain segment binding the substrate GMP of guanylate kinase differs grossly from the respective part of the adenylate kinases; it has no counterpart in the G-proteins. The binding mode of GMP is described in detail. Probably, the observed structure represents one of several structurally quite different intermediate states of the catalytic cycle.

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Related citations provided by authors (2)

  1. Three-dimensional structure of the complex of guanylate kinase from yeast with its substrate GMP.. Stehle T, Schulz GE J. Mol. Biol. 211 249-54 (1990)
  2. Guanylate kinase from Saccharomyces cerevisiae. Isolation and characterization, crystallization and preliminary X-ray analysis, amino acid sequence and comparison with adenylate kinases.. Berger A, Schiltz E, Schulz GE Eur J Biochem 184 433-43 (1989)

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