X-ray diffraction
1.9Å resolution

Histidine Ammonia-Lyase (HAL) Mutant F329G from Pseudomonas putida

Source organism: Pseudomonas putida

Function and Biology Details

Reaction catalysed:
L-histidine = urocanate + NH(3)
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
Histidine ammonia-lyase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 509 amino acids
Theoretical weight: 53.57 KDa
Source organism: Pseudomonas putida
Expression system: Escherichia coli BL21(DE3)
  • Canonical: P21310 (Residues: 2-510; Coverage: 100%)
Gene name: hutH
Sequence domains: Aromatic amino acid lyase
Structure domains:

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUB200
Spacegroup: P21
Unit cell:
a: 77.966Å b: 130.561Å c: 115.835Å
α: 90° β: 91° γ: 90°
R R work R free
0.17 not available 0.226
Expression system: Escherichia coli BL21(DE3)