1gjq

X-ray diffraction
2.7Å resolution

Pseudomonas aeruginosa cd1 nitrite reductase reduced cyanide complex

Released:

Function and Biology Details

Reactions catalysed:
NH(3) + H(2)O + acceptor = hydroxylamine + reduced acceptor
Nitric oxide + H(2)O + ferricytochrome c = nitrite + ferrocytochrome c + 2 H(+)
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nitrite reductase Chains: A, B
Molecule details ›
Chains: A, B
Length: 543 amino acids
Theoretical weight: 60.26 KDa
Source organism: Pseudomonas aeruginosa
UniProt:
  • Canonical: P24474 (Residues: 26-568; Coverage: 100%)
Gene names: PA0519, nirS
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand DHE 2 x DHE

Cofactor: Ligand HEC 2 x HEC
1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X11
Spacegroup: P21212
Unit cell:
a: 167.4Å b: 88.6Å c: 113.4Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.204 0.204 0.247