› 1gg0

X-ray diffraction
3Å resolution

CRYSTAL STRUCTURE ANALYSIS OF KDOP SYNTHASE AT 3.0 A

Released:
DOI: 10.2210/pdb1gg0/pdb
PDB entry 1gg0 coloured by chain, front view.
PDB entry 1gg0 coloured by chain, side view.
PDB entry 1gg0 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
3-Deoxy-D-manno-octulosonate-8-phosphate synthase from Escherichia coli. Model of binding of phosphoenolpyruvate and D-arabinose-5-phosphate.
Wagner T, Kretsinger RH, Bauerle R, Tolbert WD
J Mol Biol 301 233-8 (2000)
PMID: 10926505

Function and Biology Details

Reaction catalysed: 
Phosphoenolpyruvate + D-arabinose 5-phosphate + H(2)O = 3-deoxy-D-manno-octulosonate 8-phosphate + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
homo tetramer
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141505 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
2-dehydro-3-deoxyphosphooctonate aldolase Chain: A
Molecule details ›
Chain: A
Length: 284 amino acids
Theoretical weight: 30.87 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A715 (Residues: 1-284; Coverage: 100%)
Gene names: JW1206, b1215, kdsA
Sequence domains: DAHP synthetase I family
Structure domains: Aldolase class I

Ligands and Environments

1 bound ligand:
Ligand PO4 2 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: OTHER
Spacegroup: I23
Unit cell:
a: 118.167Å b: 118.167Å c: 118.167Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.231 0.227 0.265
Expression system: Escherichia coli

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Citations

1 review citation

Kdo: a critical monosaccharide for bacteria viability.
Cipolla et al. (2010)
13 other articles