› 1ga4

X-ray diffraction
1.4Å resolution

CRYSTAL STRUCTURE ANALYSIS OF PSCP (PSEUDOMONAS SERINE-CARBOXYL PROTEINASE) COMPLEXED WITH INHIBITOR PSEUDOIODOTYROSTATIN (THIS ENZYME RENAMED "SEDOLISIN" IN 2003)

Released:
DOI: 10.2210/pdb1ga4/pdb
PDB entry 1ga4 coloured by chain, front view.
PDB entry 1ga4 coloured by chain, side view.
PDB entry 1ga4 coloured by chain, top view.
Source organism: Pseudomonas sp.
Primary publication:
Carboxyl proteinase from Pseudomonas defines a novel family of subtilisin-like enzymes.
Wlodawer A, Li M, Dauter Z, Gustchina A, Uchida K, Oyama H, Dunn BM, Oda K
Nat Struct Biol 8 442-6 (2001)
PMID: 11323721

Function and Biology Details

Reaction catalysed: 
Hydrolysis of the B chain of insulin at 13-Glu-|-Ala-14, 15-Leu-|-Tyr-16 and 25-Phe-|-Tyr-26 and angiotensin I at 4-Tyr-|-Ile-5. A good synthetic substrate is Lys-Pro-Ile-Glu-Phe-|-Phe(NO(2))-Arg-Leu.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154876 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Pseudomonalisin Chain: A
Molecule details ›
Chain: A
Length: 372 amino acids
Theoretical weight: 38.45 KDa
Source organism: Pseudomonas sp.
Expression system: Escherichia coli
UniProt:
  • Canonical: P42790 (Residues: 216-587; Coverage: 63%)
Gene name: pcp
Sequence domains: Subtilase family
Structure domains: Peptidase S8/S53 domain
PSEUDOIODOTYROSTATIN Chain: I
Molecule details ›
Chain: I
Length: 3 amino acids
Theoretical weight: 522 Da
Source organism: Pseudomonas sp.
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand CA 1 x CA
Ligand GOL 1 x GOL
2 modified residues:
Ligand PHI 1 x PHI
Ligand TYB 1 x TYB

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X9B
Spacegroup: P62
Unit cell:
a: 97.63Å b: 97.63Å c: 83.3Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.122 0.118 0.155
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

5 review citations

Correlations between genotype, ultrastructural morphology and clinical phenotype in the neuronal ceroid lipofuscinoses.
Mole et al. (2005)
4 more

6 mentions without citation

Unconventional serine proteases: variations on the catalytic Ser/His/Asp triad configuration.
Ekici et al. (2008)
5 more