› 1g8x

X-ray diffraction
2.8Å resolution

STRUCTURE OF A GENETICALLY ENGINEERED MOLECULAR MOTOR

Released:
DOI: 10.2210/pdb1g8x/pdb
PDB 1g8x coloured by chain and viewed from the front.
PDB 1g8x coloured by chain and viewed from the side.
PDB 1g8x coloured by chain and viewed from the top.
Source organism: Dictyostelium discoideum
Primary publication:
Structure of a genetically engineered molecular motor.
Kliche W, Fujita-Becker S, Kollmar M, Manstein DJ, Kull FJ
EMBO J. 20 40-6 (2001)
PMID: 11226153

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alpha-actinin A; Myosin-2 heavy chain Chains: A, B
Molecule details ›
Chains: A, B
Length: 1010 amino acids
Theoretical weight: 115.21 KDa
Source organism: Dictyostelium discoideum
Expression system: Dictyostelium discoideum
UniProt:
  • Canonical: P08799 (Residues: 1-762, 763-764; Coverage: 36%)
  • Canonical: P05095 (Residues: 266-502; Coverage: 28%)
Gene names: DDB_G0268632, DDB_G0286355, abpA, actnA, mhcA
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand MG 2 x MG
Ligand ADP 2 x ADP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID13
Spacegroup: P21212
Unit cell:
a: 135.423Å b: 155.42Å c: 143.191Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.232 0.232 0.29
Expression system: Dictyostelium discoideum

Quick links

Citations

5 review citations

Synthetic biology approaches to dissecting linear motor protein function: towards the design and synthesis of artificial autonomous protein walkers.
Linke et al. (2020)
4 more

9 mentions without citation

Poorly understood aspects of striated muscle contraction.
Månsson et al. (2015)
8 more