PDBe 1g6k

X-ray diffraction
2Å resolution

Crystal structure of glucose dehydrogenase mutant E96A complexed with NAD+

Released:
Source organism: Bacillus megaterium
Entry authors: Yamamoto K, Kurisu G, Kusunoki M, Tabata S, Urabe I, Osaki S

Function and Biology Details

Reaction catalysed:
D-glucose + NAD(P)(+) = D-glucono-1,5-lactone + NAD(P)H
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assemblies composition:
homo tetramer
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glucose 1-dehydrogenase Chains: A, B, E, F
Molecule details ›
Chains: A, B, E, F
Length: 261 amino acids
Theoretical weight: 28.05 KDa
Source organism: Bacillus megaterium
Expression system: Escherichia coli
UniProt:
  • Canonical: P40288 (Residues: 1-261; Coverage: 100%)
Sequence domains: Enoyl-(Acyl carrier protein) reductase
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments


Cofactor: Ligand NAD 4 x NAD
No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-18B
Spacegroup: C2
Unit cell:
a: 121.955Å b: 66.64Å c: 120.065Å
α: 90° β: 93.44° γ: 90°
R-values:
R R work R free
0.219 0.219 0.238
Expression system: Escherichia coli