X-ray diffraction
1.8Å resolution

Structure of human DNMT2, an enigmatic DNA methyltransferase homologue


Function and Biology Details

Reaction catalysed:
S-adenosyl-L-methionine + cytosine(38) in tRNA = S-adenosyl-L-homocysteine + 5-methylcytosine(38) in tRNA
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
tRNA (cytosine(38)-C(5))-methyltransferase Chain: A
Molecule details ›
Chain: A
Length: 343 amino acids
Theoretical weight: 39.28 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
  • Canonical: O14717 (Residues: 2-391; Coverage: 88%)
  • Best match: O14717-4 (Residues: 2-58)
Gene names: DNMT2, TRDMT1
Sequence domains: C-5 cytosine-specific DNA methylase
Structure domains:

Ligands and Environments

Cofactor: Ligand SAH 1 x SAH
3 bound ligands:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12C
Spacegroup: I41
Unit cell:
a: 116.5Å b: 116.5Å c: 69.8Å
α: 90° β: 90° γ: 90°
R R work R free
0.21 0.21 0.25
Expression system: Escherichia coli