› 1g4q

X-ray diffraction
1.15Å resolution

RNA/DNA HYBRID DECAMER OF CAAAGAAAAG/CTTTTCTTTG

Released:
DOI: 10.2210/pdb1g4q/pdb
PDB entry 1g4q coloured by chain, front view.
PDB entry 1g4q coloured by chain, side view.
PDB entry 1g4q coloured by chain, top view.
Source organism: Synthetic construct
Primary publication:
Direct-methods determination of an RNA/DNA hybrid decamer at 1.15 A resolution.
Han GW
Acta Crystallogr D Biol Crystallogr 57 213-8 (2001)
PMID: 11173466

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
PDBe Complex ID:
PDB-CPX-113846 (preferred)
Entry contents:
1 distinct DNA molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
5'-D(*CP*TP*TP*TP*TP*CP*TP*TP*TP*G)-3' Chain: B
Molecule details ›
Chain: B
Length: 10 nucleotides
Theoretical weight: 2.99 KDa
Source organism: Synthetic construct
Expression system: Not provided
5'-R(*CP*AP*AP*AP*GP*AP*AP*AP*AP*G)-3' Chain: A
Molecule details ›
Chain: A
Length: 10 nucleotides
Theoretical weight: 3.26 KDa
Source organism: Synthetic construct
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand CA 3 x CA
Ligand MPD 1 x MPD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X8C
Spacegroup: P212121
Unit cell:
a: 25.699Å b: 41.067Å c: 46.113Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.143 0.143 0.186
Expression system: Not provided

Quick links

Citations

2 review citations

Recognition of the unique structure of DNA:RNA hybrids.
Shaw et al. (2008)
1 more

4 mentions without citation

Crystal structure of RNA-DNA duplex provides insight into conformational changes induced by RNase H binding.
Davis et al. (2015)
3 more