1g3k

X-ray diffraction
1.9Å resolution

CRYSTAL STRUCTURE OF THE H. INFLUENZAE PROTEASE HSLV AT 1.9 A RESOLUTION

Released:

Function and Biology Details

Reaction catalysed:
ATP-dependent cleavage of peptide bonds with broad specificity.
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo dodecamer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ATP-dependent protease subunit HslV Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 174 amino acids
Theoretical weight: 18.9 KDa
Source organism: Haemophilus influenzae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P43772 (Residues: 2-175; Coverage: 99%)
Gene names: HI_0496, hslV
Structure domains: Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-1
Spacegroup: I222
Unit cell:
a: 79.018Å b: 121.799Å c: 126.179Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.201 0.2 0.229
Expression system: Escherichia coli BL21(DE3)