X-ray diffraction
2Å resolution

Ribonuclease T1 V16C mutant

Source organism: Aspergillus oryzae
Primary publication:
Hydrophobic core manipulations in ribonuclease T1.
Biochemistry 40 10140-9 (2001)
PMID: 11513591

Function and Biology Details

Reaction catalysed:
(1a) (RNA) containing guanosine = (RNA fragment)-3'-guanosine-2',3'-cyclophosphate + a 5'-hydroxy-ribonucleotide-3'-(RNA fragment)
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Guanyl-specific ribonuclease T1 Chain: A
Molecule details ›
Chain: A
Length: 104 amino acids
Theoretical weight: 11.1 KDa
Source organism: Aspergillus oryzae
Expression system: Escherichia coli
  • Canonical: P00651 (Residues: 27-130; Coverage: 100%)
Gene names: AO090011000118, rntA
Sequence domains: ribonuclease
Structure domains: Microbial ribonucleases

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P212121
Unit cell:
a: 40.445Å b: 42.679Å c: 56.651Å
α: 90° β: 90° γ: 90°
R R work R free
0.174 0.169 0.208
Expression system: Escherichia coli