› 1fvi

X-ray diffraction
2Å resolution

CRYSTAL STRUCTURE OF CHLORELLA VIRUS DNA LIGASE-ADENYLATE

Released:
DOI: 10.2210/pdb1fvi/pdb
PDB 1fvi coloured by chain and viewed from the front.
PDB 1fvi coloured by chain and viewed from the side.
PDB 1fvi coloured by chain and viewed from the top.
Source organism: Chlorella virus
Primary publication:
Crystal structure of eukaryotic DNA ligase-adenylate illuminates the mechanism of nick sensing and strand joining.
Odell M, Sriskanda V, Shuman S, Nikolov DB
Mol. Cell 6 1183-93 (2000)
PMID: 11106756

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA ligase Chain: A
Molecule details ›
Chain: A
Length: 297 amino acids
Theoretical weight: 34.12 KDa
Source organism: Chlorella virus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A7RCB1 (Residues: 27-322; Coverage: null%)
Gene names: AR158_C658R, C658R
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand SO4 1 x SO4
Ligand AMP 1 x AMP
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P212121
Unit cell:
a: 48.17Å b: 58.44Å c: 101.79Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.19 0.19 0.27
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

15 review citations

Structural insight into DNA joining: from conserved mechanisms to diverse scaffolds.
Williamson et al. (2020)
14 more

7 mentions without citation

DNA ligases: progress and prospects.
Shuman S. (2009)
6 more