› 1fqc

X-ray diffraction
2.3Å resolution

CRYSTAL STRUCTURE OF MALTOTRIOTOL BOUND TO CLOSED-FORM MALTODEXTRIN BINDING PROTEIN

Released:
DOI: 10.2210/pdb1fqc/pdb
PDB 1fqc coloured by chain and viewed from the front.
PDB 1fqc coloured by chain and viewed from the side.
PDB 1fqc coloured by chain and viewed from the top.
Source organism: Escherichia coli
Primary publication:
Crystal structures of the maltodextrin/maltose-binding protein complexed with reduced oligosaccharides: flexibility of tertiary structure and ligand binding.
Duan X, Hall JA, Nikaido H, Quiocho FA
J. Mol. Biol. 306 1115-26 (2001)
PMID: 11237621

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Maltose/maltodextrin-binding periplasmic protein Chain: A
Molecule details ›
Chain: A
Length: 370 amino acids
Theoretical weight: 40.7 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AEX9 (Residues: 28-396; Coverage: 100%)
Gene names: JW3994, b4034, malE
Sequence domains: Bacterial extracellular solute-binding protein
Structure domains: Periplasmic binding protein-like II

Ligands and Environments

Carbohydrate polymer : NEW Components: GLO, GLC
Ligand GLO 1 x GLO
Ligand GLC 2 x GLC
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: C2
Unit cell:
a: 105Å b: 68.1Å c: 57.5Å
α: 90° β: 112.7° γ: 90°
R-values:
R R work R free
0.199 0.199 0.246
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Diversity in ABC transporters: type I, II and III importers.
Rice et al. (2014)
4 more

2 mentions without citation

The energetic cost of domain reorientation in maltose-binding protein as studied by NMR and fluorescence spectroscopy.
Millet et al. (2003)
1 more