› 1flo

X-ray diffraction
2.65Å resolution

FLP Recombinase-Holliday Junction Complex I

Released:
DOI: 10.2210/pdb1flo/pdb
PDB entry 1flo coloured by chain, front view.
PDB entry 1flo coloured by chain, side view.
PDB entry 1flo coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Crystal structure of a Flp recombinase-Holliday junction complex: assembly of an active oligomer by helix swapping.
Chen Y, Narendra U, Iype LE, Cox MM, Rice PA
Mol Cell 6 885-97 (2000)
PMID: 11090626

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero dodecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-113813 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Site-specific recombinase Flp Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 422 amino acids
Theoretical weight: 48.61 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P03870 (Residues: 2-423; Coverage: 100%)
Gene names: FLP1, R0010W
Sequence domains:
Structure domains:
SYMMETRIZED FRT DNA SITES Chains: E, G, I, K
Molecule details ›
Chains: E, G, I, K
Length: 13 nucleotides
Theoretical weight: 3.92 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Not provided
SYMMETRIZED FRT DNA SITES Chains: F, H, J, L
Molecule details ›
Chains: F, H, J, L
Length: 20 nucleotides
Theoretical weight: 6.17 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand PHS 4 x PHS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-D
Spacegroup: P21
Unit cell:
a: 81.48Å b: 180.18Å c: 98.6Å
α: 90° β: 97.02° γ: 90°
R-values:
R R work R free
0.254 0.249 0.297
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

25 review citations

Mechanisms of site-specific recombination.
Grindley et al. (2006)
24 more

9 mentions without citation

Expanding the scope of site-specific recombinases for genetic and metabolic engineering.
Gaj et al. (2014)
8 more