1fk8

X-ray diffraction
1.95Å resolution

THE CRYSTAL STRUCTURE OF THE BINARY COMPLEX WITH NAD OF 3-ALPHA-HYDROXYSTEROID DEHYDROGENASE FROM COMAMONAS TESTOSTERONI, A MEMBER OF THE SHORT CHAIN DEHYDROGENASE/REDUCTASE FAMILY

Released:

Function and Biology Details

Reaction catalysed:
A 3-alpha-hydroxysteroid + NAD(P)(+) = a 3-oxosteroid + NAD(P)H
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
3-alpha-hydroxysteroid dehydrogenase/carbonyl reductase Chains: A, B
Molecule details ›
Chains: A, B
Length: 257 amino acids
Theoretical weight: 26.42 KDa
Source organism: Comamonas testosteroni
UniProt:
  • Canonical: P80702 (Residues: 1-257; Coverage: 100%)
Gene name: hsdA
Sequence domains:
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments


Cofactor: Ligand NAD 2 x NAD
No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P1
Unit cell:
a: 42.785Å b: 46.195Å c: 64.982Å
α: 107.52° β: 106.34° γ: 97.63°
R-values:
R R work R free
0.185 0.185 0.22