1fc6

X-ray diffraction
1.8Å resolution

PHOTOSYSTEM II D1 C-TERMINAL PROCESSING PROTEASE

Released:
Source organism: Tetradesmus obliquus

Function and Biology Details

Reaction catalysed:
The enzyme shows specific recognition of a C-terminal tripeptide, Xaa-Yaa-Zaa, in which Xaa is preferably Ala or Leu, Yaa is preferably Ala or Tyr, and Zaa is preferably Ala, but then cleaves at a variable distance from the C-terminus. A typical cleavage is -Ala-Ala-|-Arg-Ala-Ala-Lys-Glu-Asn-Tyr-Ala-Leu-Ala-Ala.
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
C-terminal processing peptidase, chloroplastic Chain: A
Molecule details ›
Chain: A
Length: 388 amino acids
Theoretical weight: 41.02 KDa
Source organism: Tetradesmus obliquus
Expression system: Escherichia coli
UniProt:
  • Canonical: O04073 (Residues: 78-464; Coverage: 83%)
Gene names: D1P, ctpA
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 5ID-B
Spacegroup: C2
Unit cell:
a: 111.3Å b: 64.45Å c: 63.73Å
α: 90° β: 122.11° γ: 90°
R-values:
R R work R free
0.168 0.168 0.237
Expression system: Escherichia coli